Ian Anthony Dale as Hanzo Hasashi/Scorpion – General of the Japanese Shirai Ryu clan of ninjas, opposed to the Lin Kuei.

Harry Shum, Jr. as Kuai Liang/Sub-Zero – Lin Kuei assassin who dons the costume and identity of his older brother, Bi Han, to become the new Sub-Zero. Described as a "warlord advisor."

Brian Tee as Liu Kang – Shaolin monk and friend of Kung Lao fighting alongside Shang Tsung

Mark Dacascos as Kung Lao – Friend of Liu Kang and fellow Shaolin monk.

Casper Van Dien as Johnny Cage – Troubled, famous actor fighting alongside Earth's defenders.

David Lee McInnis as Raiden – God of Thunder and Protector of Earthrealm, leading Earth's warriors in the defence against Shang Tsung's.

Samantha Tjhia as Kitana – Former princess of Edenia, daughter of King Jerrod and Queen Sindel, raised as an assassin by Emperor Shao Kahn.

Michelle Lee as Mileena – A half-Edinian half-Tarkatan clone of Kitana, trained as Kahn's loyal assassin.

Eric Jacobus as Kurtis Stryker – Lieutenant in the Deacon City police department fighting alongside Earth's defenders.

Daniel Southworth as Kenshi – Blind swordsman possessing the power of psychokinesis.

Kim Do Nguyen as Ermac – Fighter composed of the souls of multiple dead warriors.

Cary-Hiroyuki Tagawa as Shang Tsung – Sorcerer hosting the Mortal Kombat tournament, leading his warriors against Earthrealm.

[...]

Jeri Ryan and Michael Jai White will not be returning; their replacements unknown, while Darren Shahlavi and his role as Kano will not be featured.