In the scene where Rose asserts herself as the new leader, someone makes a comment in Chinese about how people would be confused about whether she's a man or a woman. This isn't a reference to her physical sexuality though. In the original Chinese, he's actually commenting about her social behaviour, how she acts more like a man, wanting to take charge of the business and all, then all demure and shy like a woman should be. So he's accusing her of being a feminist, not a transsexual.

Also, in this scene, it's pointed out she has a 3-year old son, whom she kills to become boss. So she's obviously biologically a woman.